Testimonials

VectorBuilder is proud to share our client's testimonials:

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  All AAV AAV Serotype Testing Panel Adenovirus CRISPR/Cas9 Genome Editing Solutions Customer Service Lentivirus Library Construction Online Vector Design Plasmid DNA Preparation shRNA Gene Knockdown Solutions Technical Support Vector Cloning Vector Design Support Virus Packaging

  All AAV AAV Serotype Testing Panel Adenovirus CRISPR/Cas9 Genome Editing Solutions Customer Service Lentivirus Library Construction Online Vector Design Plasmid DNA Preparation shRNA Gene Knockdown Solutions Technical Support Vector Cloning Vector Design Support Virus Packaging

• We approached VectorBuilder with an ambitious project and stringent timelines. Ricky Nemeth exhibited remarkable responsiveness, expertise, and adaptability to our requirements. The turnaround for our request was exceptionally swift, the construction of the library precise, and we received all materials promptly. Ricky demonstrated exceptional dedication by facilitating the use of specific plate formats to deliver our viruses. I wholeheartedly recommend VectorBuilder without hesitation.

  Soledad Camolotto

  Recursion, USA

• VectorBuilder has greatly assisted our research, facilitating hypothesis testing through intramuscular injections of recombinant adeno-associated viral serotype 6 (rAAV6):Slirp vectors. The support from their team ensured that the vector met our needs, and their production methods delivered reliable results. We appreciate their valuable contribution to our research.

  Dr. Tang Cam Phung Pham

  University of Copenhagen, Denmark

• One year, over 10 constructs and transgenic fly lines further within a year, I want to thank VB and especially Joseph Perez for their excellent service. Dedicated to getting us the vectors we need, and advancing our follow-up applications- thank you for helping us advance our work!

  Jelly Soffers

  University of Missouri - Kansas City, USA

• Reliable, precise, and fast, we were well-assisted and supported on purchases, agreements, and technical aspects. For many years we have been working with Vector Builder.

  Maria Pennuto

  University of Padova, Italy

• Quality, fast and easy. I must say that VectorBuilder is the best. Very good support during all the order process as well, from the design to the shipment.

  Vitor Oliveira

  Universidade Federal de São Paulo, Brazil

• The VectorBuilder lentivirus vectors demonstrate high transduction efficiency. The team is highly professional in vector design and production.

  Yichuan Wang

  Frederick National Lab for Cancer Research, USA

• Nuestro laboratorio utiliza VB rutinariamente hace mas de 8 años. El servicio es impecable, fácil de diseñar plásmidos y con una calidad insuperable. Los envíos a Argentina son rápidos, sin trabas y con un excepcional servicio de atención al cliente en español .

  Alejandro Orlowski

  CIC-UNLP-CONICET, Argentina

• VectorBuilder is my favorite company! The service is reliable, efficient and excellent. The website user interface is intuitive. The number of choices for different vectors and downstream services is exhaustive, and the prices are reasonable. Furthermore, the team is receptive to feedback and queries. Compared to the old days of cloning, VectorBuilder now saves us enormous time and money, and frees us up to focus on the experimental questions rather than getting bogged down with making constructs.

  Dr. Timothy Cripe

  Nationwide Children’s Hospital, USA

• We chose to source AAV vectors from VectorBuilder due to the ease of online vector design, provision of expert technical support and vector production methods, which ensure high titer, purity and potency.

  Dr. George Mcilroy

  University of Aberdeen, Aberdeen, UK

• Vectorbuilder is timely, communicative, easy to work with, and—most importantly—skilled at producing very high-quality viruses. We have found that the optimal multiplicity of infection for gene-editing experiments using an AAV6 donor supplied by VectorBuilder is lower than other published reports, suggesting that the provided virus is consistently of very high potency. Knowing this saves money and time.

  Dr. Brock Roberts

  Allen Institute for Brain Research

• After ordering two plasmids from VectorBuilder, I would like to give you some feedback on the products. I am very satisfied with the plasmids sent. It's a real time saver and moreover, I had no problems concerning the expression of these plasmids in the target cells (human). The team's customer service is also attentive to requests and is very reactive regarding any problems encountered with the plasmids.

  Morgane Baldaccini

  French National Centre for Scientific Research (CNRS), France

• We have used VectorBuilder services for one year. The website is very user-friendly for designing vectors, and there are automatic verifications that make pertinent suggestions when validating sequences. The flexibility of the service is truly amazing, and we can get a perfectly characterized plasmid for the price of an antibody. Working with VectorBuilder saves us a great deal of time, and time is money. We just can't wait to get the plasmid when ordered!

  Nous utilisons les services de VectorBuilder depuis un an. Le site web est très simple à utiliser concernant le design de vecteurs et comprend des vérifications automatiques qui font des suggestions pertinentes lors de la validation des séquences. La flexibilité du service est vraiment impressionnante et nous pouvons obtenir un plasmide parfaitement caractérisé pour le prix d'un anticorps. Travailler avec VectorBuilder nous fait gagner beaucoup de temps, et le temps, c'est de l'argent. Nous sommes impatients de recevoir le plasmide lorsque nous le commandons !

  Fabien Chevalier

  French National Centre for Scientific Research (CNRS), France

• To me, VectorBuilder specially Stella Gideon has been amazing! We have gotten our plasmids made by them for over 3 years and every time they are on point! The plasmids arrive on time, and their support team is always on standby for any help I may need!

  Nemil Bhatt

  University of Texas Medical Branch

• VectorBuilder's AAV serotype panel helped us to choose the best expressing serotype for our brain area and animal model of interest. By piloting injections using a selection of serotypes provided in the panel, we obtained invaluable pilot data before ordering a large scale virus production. This allowed us to save a considerable amount of time and resources. The technical support was great, they answer our questions promptly provided great advice. VectorBuilder technical support team further help us designing and cloning our AAV viral vector. Our preliminary data allowed to map retrogradely labelled neurons in the amygdala using light-sheet microscopy. We would not hesitate to use VectorBuilder for our next projects

  Dr. Cristina Martinez Gonzalez

  University of Edinburgh, United Kingdom

• We have used VectorBuilder services for 3 years now. In our lab, we generate different types of vectors, with custom promoters and difficult sequences. I often had trouble when I synthesized these custom elements elsewhere. Going back to cloning by ourselves is no longer our first option since they are fast, efficient and their prices are more than fair. On top of that, their customer service is unbeatable.

  Dr. Gaetano Gargiulo

  Max-Delbrück-Center for Molecular Medicine (MDC), Germany

• VectorBuilder makes the process of designing and building vectors easy and efficient. Justin Mirus, as the lead, and the unique and energetic design team are promptly ready to help and support our request and advise us with their great expertise and effective services.

  Denise Panella

  Glycostem, Netherlands

• VectorBuilder is an amazing way to save time normally dedicated to molecular biology. The website is easy to use, well-conceived and allows quick design in most cases. For more unusual requests, the team is energetic, reply promptly on every request, and always finds a way to help. Low prices and short timelines really make it possible to consider VectorBuilder for many cloning matters.

  Laura Israel

  Novartis (Basel), Switzerland

• VectorBuilder offers a comprehensive online platform for vector design. We found the options for customising our vectors to be extensive and the interface simple - allowing this to be done ‘with a click’. The team has been really responsive when I wanted to check things through - thanks Christina! In my view, VectorBuilder is a platform worth investigating when you are looking to save time and outsource cloning and virus production.

  Craig Southern

  LifeArc, UK

• We find that it is more efficacious to use VectorBuilder for our molecular biology needs. Purchasing vectors and making use of cloning services frees our students’ time to concentrate on experiments. We have worked with VectorBuilder for quite some time and are always pleased with the efficient, effective, and affordable service. In particular, we are impressed by, and appreciate, how well our rep., Justin Mirus, takes care of our needs. VectorBuilder contributes money and expertise to the advancement of science, for example, to enable conferences at our institute.

  Jared Sterneckert's lab

  Center for Regenerative Therapies, Germany

• Two years ago, when another CRO was struggling to provide the requested clones that I ordered, I reached out to Vector Builder to try to make them for me. Very difficult clones to make due to many repeats of amino acids. They got the clones done for me with no issues and very quickly. I have been using Vector Builder since then. Along with this, working with Alejandro Izurieta (California Territory Manager) has been great. He is knowledgeable, and professional promptly answers my request and is courteous. I rely on him to get the service that I need from Vector Builder done. He is just wonderful!

  Irena Levin

  Takeda (California), USA

• Vectorbuilder's service is extremely consistent. We started with regular plasmids and AAV plasmids. Since last year we have ordered many piggybac plasmids. All worked as expected.

  Shuhsien Sheu

  Janelia Research Campus, USA

• We began using VectorBuilder for adenoviral plasmids construction about a year ago. We normally handled all cloning projects ourselves, but when it comes to adenoviral vectors, because of their large size, sometimes the cloning is kind of tricky for inexperienced students. So we decided to give VectorBuilder a try. The plasmid arrived one month later and the virus we generated from it expressed the correct gene. We have ordered more than 10 adenoviral plasmids and they all work fine. VectorBuilder has saved us a lot of time.

  Lei Sun

  DUKE-NUS Graduate Medical School, Singapore

• VectorBuilder’s online vector design platform is user friendly and easy to use. All one needs to do is select sequences of inserts either from their vast data base or provide one’s own unique sequences. The sequence database provides a wealth of information and links to peer-reviewed publications. Placing order for the vectors is easy and their technical and supporting staff are prompt in offering solutions. They offer services at low prices in comparison to other commercial sources. With the guaranteed service, I can concentrate on my experiments instead of constructing the vector. Thanks are especially due to Stella Gideon for her support at every step. If one is constrained with time and manpower to devote to vector construction, leave it to VectorBuilder. It is worth every penny.

  Bala Kolli

  Wayne State University, USA

• We recently purchased a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus from VectorBuilder. We have infected several tumor cell lines with excellent results. The lentiviral stocks worked very nicely, saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.

  Javier Alonso

  Research institute for Rare Diseases, Spain

• I would like to share with you my positive experience purchasing a lentiviral vector from VectorBuilder.
   
  First, I am an immunologist and have little experience with cloning. In my recent project, we needed to overexpress a gene in melanoma cells. The transfection efficiency using plasmids in this cell line was extremely low, less than 10%. Then, we tried to use antibiotics to select transfected cells and establish stably expressing cell lines. Unfortunately, for unknown reasons, the ‘stable transfectants’ lost the transfected genes! I was frustrated at this point, until I met VectorBuilder at the EACR conference in Amsterdam. Using the user-friendly website from VectorBuilder, I designed for the first time in my life my own lentiviral vector and got the product in less than one month. My group leader, who has a lot of cloning experience, could not believe that it worked so quickly. Of course, most important are the experimental results. To access the transduction efficiency, I included an EGFP tag in the vector. Now, I can transduce the melanoma cells with nearly 100% positivity. In the following assays, the genes transduced into the tumor cells resulted in a 6-fold induction in tumor immunogenicity.
  Taken together, I strongly recommend VectorBuilder to researchers, especially those who are not so experienced with cloning, and am convinced that the service and product quality from VectorBuilder can further help me to proceed with my own projects more effectively.

  Fang Zhao

  UK-Essen, Germany

• I have been consistently using VectorBuilder over the last couple of years and have been extremely satisfied with the vectors they've delivered. These include standard over-expression vectors, and Cas9 vectors on the PiggyBac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.

  Daksh Thaper

  Vancouver Prostate Centre, Canada

• VectorBuilder is the best platform for designing and synthesizing constructs. The online vector design tool is so convenient and fun to use. Ms. Joyce Zhang is always very professional in replying my questions and does it so timely. One time, something did not work out, the refund process was smooth and all the staff were very nice and professional. The constructs I asked for had two fluorescent proteins with two promotors, one has NLS and the other does not. I did transfection and it worked out very well. Joyce helped me all through this process, making my life very easy. Basically, I had many pleasant experiences with Vectorbuilder, and I strongly recommend using it. I had tried other companies, they turned out to be either slow or lacked strong technical support or are expensive. Vectorbuilder is not the cheapest, but considering the quality of its service, it is worth the money.

  Shanshan Zhu

  Johns Hopkins University, USA

• Amazing experience with VectorBuilder! I ordered plasmids at the beginning of December, and despite the holiday season they arrived exactly as scheduled. Furthermore, customer support reached out to ask what would be my desired delivery date, considering potential holiday conflicts at my institute. Overall, I am very satisfied, would highly recommend VectorBuilder (which I already do) and will buy from them again. All the best from my side!

  Tamara Tomin

  Medical University of Graz, Austria

• VectorBuilder is efficient and easy to use for the outsourcing of plasmid generations. We used the PiggBac system for transgenic modifications and we have had great success.

  Luis Cruz

  University of California San Diego, USA

• Using VectorBuilder has transformed the way I do my research. VectorBuilder’s easy-to-use web interface allows me to custom build my own plasmids, freeing up time that I would have wasted cloning and instead letting me focus on the research that really matters. They offer great prices with a fast turnaround time. Their friendly and well-informed technical support team are on hand every step of the way, should you need any help. I would strongly recommend VectorBuilder to anyone wanting to accelerate the progress of their research.

  David M Brown

  University of Nottingham, UK

• We recently purchased a number of customized plasmids on VectorBuilder. They consisted of expression vectors of two or more ORFs. Our experience was satisfactory, efficient and cost-effective.

  The designing stage was facilitated by the VectorBuilder design tool online, in which many of the most commonly used sequences, like fluorescent proteins, selection drug resistance genes, and fusion or cleavage linkers were readily offered to be picked from the ORF lists. Regarding our specific sequences, once pasted into the corresponding window of the design tool, they were immediately matched with the International Nucleotide Sequence Database Collaboration (DDBJ/EMBL/GenBank) accession numbers and compared to the databases. Where there were discrepancies, we were asked which transcripts/cDNAs we preferred to use.

  After completing the order, in a matter of three to four weeks, we had in our hands 8 of the 10 plasmids, as offered. For the rest of the plasmids we provided the template for a specific sequence. Before proceeding with the cloning, they sequenced it, detected a mutation and corrected it at a low extra cost upon our approval. A week later these corrected plasmids were delivered as well. During the whole process, VectorBuilder’s European team kept a personalized email dialogue with us, enabling us to follow-up and make decisions or accept suggestions about the plasmids.

  We have satisfactorily tested every plasmid now. We think that our decision to work with VectorBuilder has boosted our research by saving us time, resources and enabling us to concentrate more on our experiments.

  Leonel Munoz

  The Karlsruhe institute of Technology, Germany

• I have been using VectorBuilder's cloning service for several years and am extremely happy with their products and services. Their online design software is very easy to use and provides the most cutting edge solutions for today's most demanding molecular cloning needs. Their customer service is outstanding, with great technical competence and the delivery of my vectors was on time. I have saved months of precious research time by using VectorBuilder. I would recommend VectorBuilder to anyone who wants the best vector design and molecular cloning services.

  Guohao Dai

  Northeastern University, USA

• Our laboratory has had great success with both viral constructs and ready-to-use virus prepared by VectorBuilder. They provide excellent customer service at a reasonable price, and their website is extremely user-friendly. Very pleased we discovered this company.

  Marco Seandel

  Weill Cornell Medical College, USA

• We were searching for a way to outsource the cloning of our vectors and we are very happy with the service. The versatility of the tools make it a great time saver and the vectors were delivered on-time. Lentiviruses packaged are also very reliable and have been very successful in transduction studies in primary cells. Great customer service with fast response times and the team at VectorBuilder are very knowledgeable.

  Kathy Ngo

  Aridis Pharmaceuticals, USA

• We have ordered several vectors from VectorBuilder, including regular expression plasmids, targeting vectors and AAVs. The constructs were delivered on time and worked very well. They saved us a lot of time and money. Their design team pays attention to detail and made very useful suggestions. Their customer service is excellent as well. Overall, we highly recommend VectorBuilder.

  Martin Riccomagno

  University of California, Riverside, USA

• We applied CRISPR cas9 nickase in our study. VectorBuilder helped us design the gRNAs and construct plasmids with double gRNAs and double selection markers (EGFP/puromycin). The gRNA specificity is quite good, better than available online design tools. We got the knockout cell lines in the first run of electroporation with no off-target events. I would recommend VectorBuilder to CRISPR cas9 users.

  Jie Qin

  University Clinic Cologne, Germany

• I ordered a plasmid vector two years ago. The design on the web platform was easy. Contacts with the specialists to arrange further details were excellent: they were very supportive!

  Emanuela Signori

  Consiglio Nazionale delle Ricerche, Italy

• I’ve always been using VectorBuilder’s vector construction service. I’m generally satisfied with the quality of its service, and the problems I encountered were solved quickly.

  Huimin Chen

  The First Affiliated Hospital, Sun Yat-sen University, China

• We rely on VectorBuilder for high-quality molecular biology reagents. The constructs and particles are delivered to us in a timely manner. They have been more than willing to generate new plasmids and viruses if they do not work well. In summary, we have been very satisfied with VectorBuilder.

  Sathish Srinivasan

  Oklahoma Medical Research Foundation, USA

• The services provided by VectorBuilder are excellent. I had a few vector constructs made by the VectorBuilder. The description of the constructs by their software is excellent. The technical team of the company seems to have a sound knowledge and the technical suggestion regarding vector construction given to me by them were excellent.

  Saeed-ul-Hassan Khan

  Quaild-i-Azame University, Pakistan

• VectorBuilder is an amazing way to save my time for the generation of my plasmid and provide excellent downstream services. You can get any custom designed vectors you want at a very good price. I am sure I will order the vectors I need in the near future.

  Pengchao Zhang

  Houston Methodist Research Institute, USA

• I purchased a lentivirus vector and received it in less than two weeks. I am so glad that I found VectorBuilder, so I can design my lentiviral vector by picking any promotor, and from a variety of antibiotic selection markers and fluorescent tags. Upon transducing several cell lines, my target genes were highly and stably expressed with expression persisting for at least after one month after undergoing selection.

  Xianghong Chen

  H. Lee Moffitt Cancer Center & Research Institute, USA
  

• VectorBuilder has provided very satisfactory service in making various AAV vector constructs for us. The new sales representative is very proactive in offering help on explaining and introducing other VectorBuilder service to us. Expecting to get more support and service from VectorBuilder.

  Qingchun Tong

  University of Texas Medical School at Houston, USA

• I’ve used VectorBuilder’s vector construction and lentivirus packaging services. The turnaround time was short and everything worked well. Also, I received quick responses from the customer support team.

  Xijuan Chen

  Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, China

• VectorBuilder has helped with my research progress. Cloning hasn't been an issue in our lab until recently. Their construct worked well. They saved me a lot of time and provide great services!

  Jennifer Cho

  University of California, Riverside, USA

• I would like to share with you all my experience with VectorBuilder. I do a lot of vector designs for different applications. VectorBuilder website is easy to use and offers efficient and fast vector design tools. In addition, VectorBuilder team is friendly and is open to accommodate special custom and bespoke requirements for vector modifications that are not readily available on the website. Finally, the price is more than reasonable, and I would recommend checking VectorBuilder website for cloning projects.

  Yaron Galanty

  Wellcome Trust/CRUK Gurdon Institute, University of Cambridge, UK

• We ordered vectors from Vectorbuilder already many times. We are happy with the service. The vectors work well and service is fast. I would highly recommend Vectorbuilder. We have very good experience with them.

  Rene Opavsky

  University of Florida, USA

• The scientific standards of VectorBuilder’s services are unquestionably high. The web interface to design vectors is intuitive and in fact so easy to use that it can even serve for educational purposes. VectorBuilder has pretty much covered everything one can think about when wanting to design an expression vector, it’s great to have all the versatility on one page. Turnaround time is fast and owing to their professionalism and customer friendly attitude I can recommend VectorBuilder’s services without reservation.

  Johnny Kim

  Max Planck Institute for Heart and Lung Research, Germany

• We ordered adenovirus from VectorBuilder to overexpress our protein of interest in primary mouse hepatocytes. The ordering process, including vector design, was very fast and easy. We received the plasmid and adenovirus within the time frame that was stated during the ordering process and the transduction efficiency of the adenovirus was very high, enabling us to use a low MOI. As a bonus, we also received the control Ad-EGFP adenovirus for free. In the future, I will not use any more siRNA or classical transfection methods on primary mouse hepatocytes, just adenoviruses from VB! I can highly recommend VectorBuilder's services for vector design and adenovirus production.

  Alexander Mayer

  University of Würzburg, Germany

• We have used VectorBuilder for multiple vectors including regular, lentiviral, Tet-inducible, and CRISPR/Cas9 vectors. Designing the vectors is super easy and we have had good luck with them. I would definitely recommend their products!

  Steven Wall

  Texas A&M University, USA